David Galbraith
Professor, School of Plant Sciences
520-621-9153

David Galbraith obtained undergraduate and graduate degrees in Biochemistry from the University of Cambridge, and postdoctoral training as a NATO Fellow at Stanford University. His first academic appointment was at the University of Nebraska Lincoln, and he became Professor of Plant Sciences at the University of Arizona in 1989. His research has focused on the development of instrumentation and methods for the analysis of biological cells, organs, and systems. He pioneered the use of flow cytometry and sorting in plants, developing widely-used methods for the analysis of genome size and cell cycle status, and for the production of somatic hybrids. He also was amongst the first to develop methods for the analysis of gene expression within specific cell types, using markers based on Fluorescent Protein expression for flow sorting these cells, and microarray platforms for analysis of their transcriptional activities and protein complements. Current interests include applications of highly parallel platforms for transcript and protein profiling of minimal sample sizes, and for analysis of genetic and epigenetic mechanisms that regulate gene expression during normal development and in diseased states. He has published more than 170 scholarly research articles, holds several patents, and was elected a Fellow of the American Association for Advancement of Science in 2002.

Selected Citations: 

Galbraith, D.W., Harkins, K.R., Maddox, J.R., Ayres, N.M., Sharma, D.P., and Firoozabady, E. (1983). Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220:1049-1051. Afonso, C.L., Harkins, K.R., Thomas-Compton, M., Krejci, A., and Galbraith, D.W. (1985). Production of somatic hybrid plants through fluorescence activated sorting of protoplasts. Nature Biotechnology 3:811-816. Harkins, K.R., and Galbraith, D.W. (1987). Factors governing the flow cytometric analysis and sorting of large biological particles. Cytometry 8:60-71. Harkins, K.R., Jefferson, R.A., Kavanagh, T.A., Bevan, M.W., and Galbraith, D.W. (1990). Expression of photosynthesis related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts. Proceedings of the National Academy of Sciences U.S.A. 87:816-820. Bharathan, G., Lambert, G.M., and Galbraith, D.W. (1994). Nuclear DNA contents of monocotyledons and related taxa. American Journal of Botany 81:381-386. Zilmer, N.A., Rodriguez, J.J., Yopp, T.A., Lambert, G.M., and Galbraith, D.W. (1995). Flow cytometric analysis using digital signal processing. Cytometry 20:102-117. Galbraith, D.W., Grebenok, R.J., Lambert, G.M., and Sheen, J. (1995). Flow cytometric analysis of transgene expression in higher plants: Green Fluorescent Protein. Methods in Cell Biology 50:3-12. Sheen, J., Hwang, S., Niwa, Y., Kobayashi, H., and Galbraith, D.W. (1995). Green fluorescent protein as a new vital marker in plant cells. Plant Journal 8:777-784. Gong, F.-C., Giddings, T.H., Meehl, J.B., Staehelin, L.A., and Galbraith, D.W. (1996). Z-membranes: artificial organelles for over-expressing recombinant integral membrane proteins. Proceedings of the National Academy of Sciences U.S.A. 93:2219-2223. Grebenok, R.J., Pierson, E.A., Lambert, G.M., Gong, F.-C., Afonso, C.L., Haldeman-Cahill, R., Carrington, J.C., and Galbraith, D.W. (1997). Green-Fluorescent Protein fusions for efficient characterization of nuclear localization signals. Plant Journal 11:573-586. Grebenok, R.J., Lambert, G.M., and Galbraith, D.W. (1997). Characterization of the targeted nuclear accumulation of GFP within the cells of transgenic plants. Plant Journal 12:685-696. Winkler, R.G., Frank, M.R., Galbraith, D.W., Feyereisen, R., and Feldmann, K.A. (1998). Systematic reverse genetics of Transfer-DNA-tagged lines of Arabidopsis: isolation of mutations in the cytochrome P450 gene superfamily. Plant Physiology 118:743-750. Chytilova, E., Macas, J., Sliwinska, E., Rafelski, S., Lambert, G., and Galbraith, D.W. (2000). Nuclear dynamics in Arabidopsis thaliana. Molecular Biology of the Cell 11:2733-2741. Kawasaki, S., Borchert, C., Deyholos, M., Wang, H., Brazille, S., Kawai, K., Galbraith, D.W., and Bohnert, H.J. (2001). Gene expression profiles during the initial phase of salt stress in rice (Oryza sativa L.). Plant Cell 13:889-906. Xu, W., Bak, S., Decker, A., Paquette, S.M., Feyereisen, R., and Galbraith, D.W. (2001). Microarray-based analysis of gene expression in very large gene families: the Cytochrome P450 gene superfamily of Arabidopsis thaliana. Gene 272:61-74. Zhang, X., Zhang, L., Dong, F., Gao J., Galbraith, D.W., and Song, C.-P. (2001). Hydrogen peroxide is involved in abscisic acid-induced stomatal closure in Vicia faba. Plant Physiology 126:1438-1448. Birnbaum, K., Shasha, D.E., Wang, J.Y., Jung, J.W., Lambert, G.M., Galbraith, D.W., and Benfey, P.N. (2003). A gene expression map of the Arabidopsis root. Science 302:1956-1960. Song, C-P., Guo, Y., Qiu, Q., Lambert, G., Galbraith, D.W., Jagendorf, A, and Zhu, J.-K. (2004). A probable Na+/H+ exchanger on the chloroplast envelope functions in pH homeostasis and chloroplast development in Arabidopsis thaliana. Proc. Natl. Acad. Sci. U.S.A. 101:10211-10216. Kristensen, C., Morant, M., Olsen, C.E., Galbraith, D.W., Lindberg-Møller, B., and Bak, S. (2005). Metabolic engineering of dhurrin in transgenic Arabidopsis plants with marginal inadvertent effects on the metabolome and transcriptome. Proc. Natl. Acad. Sci. U.S.A. 102:1779-1884. Zanetti, M.E., Chang, I.-F., Gong, F.C., Galbraith, D.W., and Bailey-Serres, J. (2005). Immunopurification of polyribosomal complexes of arabidopsis for global analysis of gene expression. Plant Physiology 138:624-635. Birnbaum, K., Jung, J.W., Wang, J.Y., Lambert, G.M., Hirst, J.A., Galbraith, D.W., and Benfey, P.N. (2005). Cell-type specific expression profiling in plants using fluorescent reporter lines, protoplasting, and cell sorting. Nature Methods 2:1-5. LaJeunesse, T.C., Lambert, G., Andersen, R.A., Coffroth, M.A., and Galbraith, D.W. (2005). Symbiodinium (Pyrrhophyta) genome sizes (DNA content) are smallest among dinoflagellates. Journal of Phycology 41:880-886. Zhang, C.Q, Gong, F.C., Lambert, G.M., and Galbraith, D.W. (2005). Cell type-specific characterization of nuclear DNA contents within complex tissues and organs. Plant Methods 2005, 1:7 doi:10.1186/1746-4811-1-7. Song, C-P., and Galbraith, D.W. (2006). AtSAP18, an orthologue of human SAP18, is involved in the regulation of salt stress and mediates transcriptional repression in Arabidopsis. Plant Molecular Biology 60:241-257. Rangwala, S.H., Elumalai, R., Vanier, C., Ozkan, H., Galbraith, D.W., and Richards, E.J. (2006). Meiotically-stable natural epigenetic variation in Sadhu, a novel Arabidopsis retroposon. P.L.o.S. Genetics 2:270-281. Li, J., Li, X., Su, H., Chen, H., and Galbraith, D.W. (2006). A framework of integrating gene relations from heterogeneous data sources: an experiment on Arabidopsis thaliana. Bioinformatics 22:2037-2043. Galbraith, D.W. (2006). The daunting process of MIAME. Nature 444:31. Kris, R.M., Felder, S., Deyholos, M., Lambert, G.M., Botros, I., Martel, R., Seligmann, B., and Galbraith, D.W. (2007). High-throughput, high-sensitivity analysis of gene expression in Arabidopsis thaliana. Plant Physiology 144:1256-1266. Barthelson, R.A., Lambert, G.M., Vanier, C., Lynch, R.M., and Galbraith, D.W. (2007). Comparison of the contributions of the nuclear and cytoplasmic compartments to global gene expression in human cells. B.M.C. Genomics 8:340. Zhang, C.Q., Barthelson, R.A., Lambert, G.M., and Galbraith, D.W. (2008). Characterization of cell-specific gene expression through fluorescence-activated sorting of nuclei. Plant Physiology 147:30-40. Edwards, J.D., Sweeney, M., Janda, J., Gaikwad, A., Liu, B., Leung, H., and Galbraith, D.W. (2008). Development of a high-throughput, low-cost genotyping platform based on oligonucleotide microarrays, and its evaluation in rice. Plant Methods 2008, 4:13. Galbraith, D.W. (2009). Simultaneous flow cytometric quantification of plant nuclear DNA contents over the full range of described angiosperm 2C values. Cytometry 75A:692-698. Mustroph, A., Zanetti, M.E., Jang, C.J.H., Galbraith, D.W., Girke, T., and Bailey-Serres, J. (2009). Profiling translatomes of discrete cell populations resolves altered cellular priorities during hypoxia in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A. 106:18849-18854. Zárate, X., Henderson, D.C., Phillips, K.C., Lake, A.D., and Galbraith, D.W. (2010). Development of high-yield autofluorescent protein microarrays using hybrid cell-free expression with combined Escherichia coli S30 and wheat germ extracts. Proteome Science 2010, 8:32 doi:10.1186/1477-5956-8-32. Liu, Y., Zhu, X.Y., Zhang, S., Bernardo, M., Edwards, J.D., Galbraith, D.W., Leach, J., Zhang, G., Liu, B., and Leung, H. (2011). Dissecting quantitative resistance against blast using heterogeneous inbred families in rice. Theoretical and Applied Genetics 122:341-353. Galbraith, D.W., Janda, J., and Lambert, G.M. (2011). Multiparametric analysis, sorting, and transcriptional profiling of plant protoplasts and nuclei according to cell type. Methods in Molecular Biology 699:407-429. Krishnan, A.R., Sweeney, M., Vasic, J., Galbraith, D.W., and Vasic, B. (2011). Barcodes for DNA sequencing with guaranteed error correction capability. Electronics Letters 47:236-237. Bourzac, K.M., Rounseville, M.P., Zarate, X., Maddula, V.S.R.K., Henderson, D.C., Luckey, J., Seligmann, B., and Galbraith, D.W. (2011). A high-density quantitative nuclease protection microarray platform for high throughput analysis of gene expression. Journal of Biotechnology 154:68-75. Kimzey, M.J., Zarate, X., Galbraith, D.W. and Lau, S.S. (2011). Optimizing microarray-based in situ transcription-translation of proteins for MALDI mass spectrometry. Analytical Biochemistry 414:282–286. Galbraith, D.W. (2011). Frontiers in Genomic Assay Technologies: the grand challenges in enabling data-intensive biological research. Frontiers in Genetics 2:26. doi: 10.3389 / fgene.2011.00026. Galbraith, D.W., Bennetzen, J.L., Kellogg, E.A., Pires, J.C., and Soltis, P.S. (2011). The genomes of all angiosperms: a call for a coordinated global census. Journal of Botany Article 646198 doi:10.1155/2011/646198. Baisakh, N., Ramana Rao, M. V., Rajasekaran, K., Subudhi, P., Janda, J., Galbraith, D., Vanier, C. and Pereira, A. (2012). Enhanced salt stress tolerance of rice plants expressing a vacuolar H+-ATPase subunit c1 (SaVHAc1) gene from the halophyte grass Spartina alterniflora Löisel. Plant Biotechnology Journal 10:453–464. Zhang, C.Q., and Galbraith, D.W. (2012). RNA interference-mediated gene knockdown within specific cell types. Plant Molecular Biology 80:169-176. Galbraith, D.W. (2012). Flow Cytometry and Cell Sorting: the Next Generation (guest volume editor). Methods Volume 57. Wang, Z., Hobson, N., Galindo, L., McDill, J., Hawkins, S., Neutelings, G., Datla, R., Lambert, G., Galbraith, D.W., Cullis, C., Wong, G.K.-S., Wang J., and Deyholos, M.K. (2012). The genome of flax (Linum usitatissimum) assembled de novo from short shotgun sequence reads. Plant Journal 14:461-473. Qin, J., Scheuring, C.F., Wei, G., Zhi, H., Zhang, M., Huang, J.J., Zhou, X., Galbraith, D.W., and Zhang, H.-B. (2013). Identification and characterization of a repertoire of genes differentially expressed in developing top ear shoots between a superior hybrid and its parental inbreds in Zea mays L. Molecular Genetics and Genomics 288:691-705. Grindberg, R.V., Yee-Greenbaum, J.L., McConnell, M.J., Novotny, M., O’Shaughnessy, A.L., Lambert, G.M., Araúzo-Bravo, M.J., Lee, J., Fishman, M., Lin, X., Robbins, G.E., Lin, X., Venepally, P., Badger, J.H., Galbraith, D.W., Gage, F.H., and Lasken, R.S. (2013). RNA-Seq from single nuclei. Proceedings of the National Academy of Sciences U.S.A. 110:19802-19807. Galbraith, D.W. (2014). Flow cytometry and sorting in Arabidopsis. In: Arabidopsis Protocols (3rd. edition; J.J.S. Serrano, ed.). Methods in Molecular Biology 1062:509-537. Galbraith, D.W. (2014). Endoreduplicative standards for calibration of flow cytometric C-value measurements. Cytometry 85A:368-374. Chen, S.-C., Cannon, C.H., Kua, C.-S., Liu, J.J., and Galbraith, D.W. (2014). Genome size variation in the Fagaceae and its implications for trees. Tree Genetics and Genomes 10:977-988. Mittal, A., Balasubramanian, R., Cao, J., Singh, P., Subramanian, S., Hicks, G., Nothnagel, E.A., Abidi, N., Janda, J., Galbraith, D.W., and Rock, C.D. (2014). TOPOISOMERASE 6B is involved in chromatin remodeling associated with hormone and environmental control of carbon partitioning, secondary metabolite and cell wall synthesis, and epidermal morphogenesis in Arabidopsis. Journal of Experimental Botany 65:4217-4239. Galbraith, D.W. (2014). Challenges and solutions in cytometric measurements of non-mammalian species. Cytometry 85A:831-832. Zarate, X., and Galbraith, D.W. (2014). A cell-free expression platform for production of protein microarrays. Methods in Molecular Biology 1118:297-307.

Research Themes: 
Climate and Adaptation
Food Systems and Security
Natural Environment and Biodiversity
Agriculture
Conservation biology
Genetics
Plant ecology
Plant science
Agriculture
International development
Plant science
Agriculture
Arid lands

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